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Screening for viruses infecting chrysanthemum cultivars in India.

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Title Screening for viruses infecting chrysanthemum cultivars in India.
 
Creator Verma, N
Mehra, A
Singh, L
Hallan, Vipin
Singh, A K
Jabeen, N
Singh, M K
Raja, Ram
Zaidi, A A
 
Subject Plant sciences
 
Description Screening of chrysanthemum cultivars was carried out using enzyme linked immunosorbent assay (ELISA), northern hybridization and reverse transcription polymerase chain reaction (RT-PCR) for Chrysanthemum B (CVB), Cucumber mosaic (CMV), Tomato aspermy (TAV), tospo- and potyviruses. By hybridization and RT-PCR, 48.7%, 42.5%, 26.2%, 12.5% and 6.25% cultivars were found to be infected with CVB, CMV, TAV, tospo- and potyviruses, respectively out of 80 cultivars tested. CVB was the most prevalent virus while infections by poty- and tospoviruses were found less frequently. The percentage of the single virus infection in chrysanthemum cultivars under study was the highest (35%) followed by double (26.25%), triple (8.75%) and for tetra and pentavirus infection it was 2.5%. Nucleic acid based detection techniques were found to be more reliable and sensitive than ELISA.
 
Publisher Elsevier Science BV, PB No.211, 1000 AE Amsterdam, The Netherlands.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/185/1/chrysanthemum.pdf
Verma, N and Mehra, A and Singh, L and Hallan, Vipin and Singh, A K and Jabeen, N and Singh, M K and Raja, Ram and Zaidi, A A (2007) Screening for viruses infecting chrysanthemum cultivars in India. Scientia Horticulture, 111 (3). pp. 260-265. ISSN 0304-4238
 
Relation http://ihbt.csircentral.net/185/