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First report of Carnation necrotic fleck virus (CNFV) infecting carnations in India

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Title First report of
Carnation necrotic fleck virus
(CNFV) infecting
carnations in India
 
Creator Hallan, Vipin
Raikhy, G
kulshrestha, S
Sharma, M L
Raja, Ram
Zaidi, A A
 
Subject Plant viruses
 
Description In a survey of carnations grown in the Kangra valley
of Himachal Pradesh, India, 17 out of the 35 varieties
inspected showed mottling with grey streaks or red
necrotic flecks. These symptoms were similar to those
previously reported for
Carnation necrotic fleck virus
(CNFV) (Bar-Joseph
et al.
, 1979). Sap from leaves with
symptoms was inoculated on to
Dianthus caryophyllus
,
D. chinensis
and
D. barbatus.
Symptoms produced on
these
Dianthus
spp. indicator plants included mottling,
vein clearing, flecking, grey streaks or red necrotic
flecks. No symptoms were observed when
Chenopodium
spp. were inoculated. Using a polyclonal DAS-ELISA kit
(Agdia Inc., Elkhart, IN, USA), CNFV was detected in
Dianthus
indicator plants and the following carnation
varieties: Arthur Sim, Cabarett, Charmour, Dessio, Dusty
Pink, Espana, Flair, Irma, Josh, LasPama, New Espana,
Orange Triumph, Prinidello, Red Carso, Scania, Shocking
Pink and White Candy. The virus was transmitted in a
semipersistent manner by aphid species
Myzus persicae
,
Aphis craccivora
and
Aphis gossypii
from
D. caryophyllus
to
D. barbatus
, with infection confirmed by DAS-ELISA.
When examined by transmission electron microscopy,
leaf dip preparations showed flexuous virus particles
1400–1500 nm long. Reverse transcriptase-polymerase
chain reaction (RT-PCR) was conducted using degenerate
primers specific to the closterovirus group (Karasev
et al.
,
1994) and an amplicon of the expected size (
∼
1000 bp)
was generated. All these tests identified the virus as
CNFV. This virus has been reported from many countries,
including the USA, France, Italy and Yugoslavia, but
this is the first report of CNFV infecting carnations in
India.
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/193/1/CNFV.pdf
Hallan, Vipin and Raikhy, G and kulshrestha, S and Sharma, M L and Raja, Ram and Zaidi, A A (2003) First report of Carnation necrotic fleck virus (CNFV) infecting carnations in India. Plant Pathology, 52. p. 801.
 
Relation http://ihbt.csircentral.net/193/