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Isolation and Identification of a Novel Strain of Pseudomonas chlororaphis Capable of Transforming Isoeugenol to Vanillin

IR@CSIR-IHBT

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Title Isolation and Identification of a Novel Strain of Pseudomonas chlororaphis Capable of Transforming Isoeugenol to Vanillin
 
Creator Kasana , Ramesh C
Sharma , U K
Sharma , N
Sinha, Arun K
 
Subject Microbiology
 
Description Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the
world. Taking into consideration the worldwide demand for natural vanillin and its limited supply,
alternative routes for its production including biotransformation are being constantly explored. In this
regard, a novel soil bacterium capable of converting isoeugenol to vanillin was isolated by conventional
enrichment process from soils of Ocimum field. On the basis of morphological and physiochemical
characteristics and 16S rRNA gene sequence analysis, the isolate was identified as Pseudomonas
chlororaphis CDAE5 (EMBL # AM158279). Vanillin formation was analyzed by gas chromatography
(GC), and its structure was confirmed by GC-mass spectrometry and nuclear magnetic resonance. After
24-h reaction, the vanillin concentration reached 1.2 g L)1 from 10 g L)1 isoeugenol in 20-mL reaction
solution at 25 degree C and 180 rpm. The strain showed potential to be a good candidate for biotechnological
production of vanillin from isoeugenol. Further studies for standardization and optimization for higher
yield of vanillin production needs to be investigated.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/195/1/currmicro.pdf
Kasana , Ramesh C and Sharma , U K and Sharma , N and Sinha, Arun K (2007) Isolation and Identification of a Novel Strain of Pseudomonas chlororaphis Capable of Transforming Isoeugenol to Vanillin. Current Microbiology, 54. pp. 457-461.
 
Relation http://ihbt.csircentral.net/195/