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Induction of apoptosis by a synergistic lignand composition from Cedrus deodarain human cancer cells

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Title Induction of apoptosis by a synergistic lignand composition from Cedrus deodarain human cancer cells
 
Creator Sharma, Parduman R.
Shanmugavel, M.
Saxena, Ajit K.
Qazi, Ghulam N.
 
Subject Pharmacological Sciences
 
Description AP9-cd, a synergistic lignan mixture from Cedrus deodara (Pinaceae) consisting of (−)-wikstromal, (−)-matairesinol
and dibenzyl butyrolactol, depicted cytotoxic effects against numerous human cancer cell lines reported previously.The aim of this study was to investigate the mechanism of cell death in human cancer cells. The viability, morphological and ultrastructural changes in Molt-4 cells were investigated. Using the trypan blue
exclusion assay, we demonstrated that AP9-cd significantly reduced the viability of Molt-4 cells in a time- and
dose-dependent manner. Apoptotic assays using light microscopy revealed that this agent induced Molt-4 cell
apoptosis at varied concentrations. The treatment causes a loss in cell viability by activating the apoptotic
process as identified by light and electron microscopy. The morphological changes of intracellular organelles in Molt-4 cells treated with 30 μg/ml of AP9-cd revealed the disruption of mitochondrial cristae. Other features
included the vacuolization, chromatin condensation and formation of micronuclei. Surface ultrastructural
studies of four different tumor cell lines (Molt-4, HL-60, PC-3 and A-549) treated with AP9-cd depicted loss
of surface projections, condensation and formation of apoptotic bodies. AP9-cd treatment to transgenic fruit
fly, Drosophila, carrying human adenomatous polpyposis coli (hAPC) gene enhanced eye phenotypes and therefore may inhibit Wnt/Wg pathway which is important in the aetiology of a number of human cancers.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/277/1/10.1002ptr.2511.pdf
Sharma, Parduman R. and Shanmugavel, M. and Saxena, Ajit K. and Qazi, Ghulam N. (2008) Induction of apoptosis by a synergistic lignand composition from Cedrus deodarain human cancer cells. Phytotherapy Research, 22 (12). pp. 1587-1594. ISSN 0951418X
 
Relation http://dx.doi.org/10.1002/ptr.2511
http://iiim.csircentral.net/277/