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Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile

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Title Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile
 
Creator Verma, Monika
Singh, Shashank
Bhushan, Shashi
Pal, Harish
Kitchlu, Surinder
Koul, Maharaj
Thappa, Rajender
Saxena, Ajit
 
Subject Biological Sciences
Pharmacological Sciences
 
Description The essential oil of Tanacetum gracile (Accession
no. AT-01 termed AT-01 in the manuscript), a cold desert alpine highly aromatic herb, has 40 constituents including lavendulol (21.5%), lavendulol acetate (1.7 %), α-pinene (11.2%),1,8-cineole (15.2 %), cis-β-ocimene (6.9 %), borneol (6.1%), limonene (5.1%) and chamazulene (3.7 %). AT-01 was evaluated for its anticancer activity. It inhibited HL-60 cell proliferation with an IC50 of 27 μg/
mL. Furthermore, AT-01 induced apoptosis in human
leukemia HL-60 cells as measured by several biological end points. AT-01 induced apoptotic body formation, enhanced annexinV-FITC binding of the cells, increased sub-G0 DNA fraction, loss of mitochondrial membrane potential (Δψmt) and release of cytochrome c from mitochondria, activated
caspase-9 as well as caspase-3, and increased cleavage of PARP in HL-60 cells. Thus, AT-01 induced apoptosis through the mitochondrial dependant pathway in HL-60 cells.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/278/1/10.1055s-2008-1074503.pdf
Verma, Monika and Singh, Shashank and Bhushan, Shashi and Pal, Harish and Kitchlu, Surinder and Koul, Maharaj and Thappa, Rajender and Saxena, Ajit (2008) Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile. Planta Medica, 74 (5). pp. 515-520. ISSN 0032-0943
 
Relation http://dx.doi.org/10.1055/s-2008-1074503
http://iiim.csircentral.net/278/