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Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity

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Title Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity
 
Creator Kaur, Amandeep
Singh, Jatinder
Kamboj, Sukhdev Singh
Sexana, A.K.
Pandita, Renu Moti
Shamnugavel, M.
 
Subject Chemical Sciences
 
Description A lectin with antiproliferative activity towards human cancer cell lines and mitogenic towards human peripheral blood mononuclear cells was purified from the rhizomes of Arundo donax (Linn.) by affinity chromatography on N-acetyl-D-glucosamine linked to epoxy-activated sepharose-6B. The pure preparation apparently yielded a single band of approximately 15 kDa on SDS–PAGE,pH 8.3, under both reducing and non-reducing conditions. The molecular mass of native lectin was 32 kDa as determined by gel filtration chromatography. This showed the lectin to be a dimer, with subunits not held together by disulphide linkages. The A.
donax lectin (ADL) agglutinated rabbit erythrocytes and the agglutination was inhibited by N-acetyl-D-glucosamine and its diand trimer. The lectin was thermostable upto 55 �C and showed optimum activity in the range of pH 7.0–9.0 and comprised of 2.1% carbohydrate content.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/282/1/10.1016j.phytochem.2005.06.026.pdf
Kaur, Amandeep and Singh, Jatinder and Kamboj, Sukhdev Singh and Sexana, A.K. and Pandita, Renu Moti and Shamnugavel, M. (2005) Isolation of an N-acetyl-d-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity. Phytochemistry, 66 (16). pp. 1933-1940. ISSN 00319422
 
Relation http://dx.doi.org/10.1016/j.phytochem.2005.06.026
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