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Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae

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Title Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae
 
Creator Rehman, Shakil
Khan, Reehana
Bhat, Khursheed A.
Raja, Alsaba F.
Shawl, Abdul S.
Alam, Mohd S.
 
Subject Biological Sciences
 
Description RESUMO: “Estudos de isolamento, caracterização e atividade antibacteriana de cumarinas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae”. Seis cumarinas dafinina (1), dafinetina (2), dafinetina glicosídeo (3), rodonetina (4), rodonina (5) eumbeliferona (6) foram isoladas do extrato metanólico das partes aéreas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Os compostos e seus derivados acetilados foram testados para verificar sua atividade antibacteriana contra Staphylococcus aureus ATCC-29213,
Escherichia coli resistente à meticilina, Staphylococcus aureus ATCC-15187, ATCC-8739,Pseudomonas aeruginosa ATCC-9027, pelo método de microdiluição, usando ciprofloxacina como referência. A substância 2 apresentou a melhor atividade antibacteriana com o MIC 125 μg/mL contra S. aureus ATCC-29213 e MRSA ATCC-15187 seguido pela substância 4, que apresentou o valor de CIM de 250 μg/mL contra as quatro cepas testadas. Todas as moléculas
apresentaram melhor atividade antibacteriana do que seus derivados acetilados.
Unitermos: Rhododendron lepidotum, glicosídeos cumarínicos, HMBC, antibacteriano, daphnin,
daphnetin.
ABSTRACT: Six coumarins daphnin (1), daphnetin (2), daphnetin glucoside (3), rhodonetin (4), rhodonin (5) and umbelliferone (6) were isolated from the methanolic extract of Rhododendron lepidotum Wall. ex G. Don, Ericaceae (aerial part). The compounds and their acetyl derivatives were screened for antibacterial activity against Staphylococcus aureus ATCC-29213, methicillin resistant Staphylococcus aureus ATCC-15187, Escherichia coli
ATCC-8739, Pseudomonas aeruginosa ATCC-9027 by microdilution method as compared to the reference ciprofloxacin. Compound 2 displayed the best antibacterial activity with MIC 125 μg/mL against S. aureus ATCC-29213 and MRSA ATCC-15187 followed by 4 which exhibited the MIC value of 250 μg/mL against all the four tested strains. All molecules showed better antibacterial activity than their acyl derivatives.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/283/1/10.1590S0102-695X2010005000037.pdf
Rehman, Shakil and Khan, Reehana and Bhat, Khursheed A. and Raja, Alsaba F. and Shawl, Abdul S. and Alam, Mohd S. (2010) Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Revista Brasileira de Farmacognosia, 20 (6). pp. 886-890. ISSN 0102-695X
 
Relation http://dx.doi.org/10.1590/S0102-695X2010005000037
http://iiim.csircentral.net/283/