Record Details

Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC.

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC.
 
Creator Singh, N
Singh , Bikram
Gupta , A P
Kaul , V K
 
Subject Natural Product Chemistry
 
Description A new high performance thin layer chromatography (HPTLC) method for
the simultaneous quantification of picroside-I and picroside-II in P. kurroa is
described. Separation of picroside-I and picroside-II was achieved by mobile phase
of CHCl3:MeOH (82:18, v/v) on precoated silica gel 60 F254 aluminum plate. The
densitometric determination of picrosides was carried out at 290 nm, in absorptionreflection
mode. The calibration curves were linear in the range of (2–5mg). The
method is simple, specific, rapid, and reliable for simultaneous determination of P-I
and P-II in P. kurroa. The proposed method was applied for accurate quantification
of large number of samples collected from different altitudes of western Himalaya.
 
Publisher Taylor & Francis Inc , 325 Chestnut St, Suite 800, Philadelphia, PA 19106 USA
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/217/1/JLCRT1.pdf
Singh, N and Singh , Bikram and Gupta , A P and Kaul , V K (2005) Quantification of Picroside-I and Picroside-II in picrorhiza kurroa by HPTLC. Journal of Chromatography and Related Technologies, 28 (11). pp. 1679-1691. ISSN 1082-6076
 
Relation http://ihbt.csircentral.net/217/