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Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates from India

IR@CSIR-IHBT

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Title Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates
from India
 
Creator Hallan, Vipin
Sherpa , A R
Pathak, P
Zaidi, A A
 
Subject Plant viruses
 
Description The variability in the coat protein (CP) gene sequence
of Cymbidium mosaic virus (CymMV) that naturally
infects orchids worldwide was investigated. Samples
were collected from different regions of India, and the
gene encoding the CP of nine isolates was specifically
amplified by reverse-transcription polymerase chain
reaction. The amplified product obtained was cloned,
sequenced and multiple sequence alignment of deduced
amino acid (aa) sequences revealed considerable
homology to CymMV isolates from other countries.
The nucleotide sequences and the amino acid
sequences were found to be 85–100% identical and
65–100% respectively. Such high sequence conservation
suggests that the CymMV CP gene is highly conserved
and is a suitable candidate for the development
of diagnostic procedures and to provide transgenic
resistance to orchids cultivated in different geographical
locations. Although recombination is not common
among CymMV isolates, one isolate from Cymbidium
was found to be a recombinant between a Korean and
a Thai isolate of the virus. IHBT communication no:
0451.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/219/1/jph_1093.pdf
Hallan, Vipin and Sherpa , A R and Pathak, P and Zaidi, A A (2006) Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates from India. Journal of Phytopathology, 154 (5). pp. 275-280.
 
Relation http://ihbt.csircentral.net/219/