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Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue

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Title Differential expression of Histone H3 gene in tea (Camellia
sinensis (L.) O. Kuntze) suggests its role in growing tissue
 
Creator Singh, K
Kumar, Sanjay
Ahuja, Paramvir Singh
 
Subject Plant sciences
Nutraceuticals
 
Description Histone proteins are integral part of chromatin
and their expression is typically linked toDNAreplication in
the S phase of cell cycle. Histone H3 is one of the four
histones, along with H2A, H2B and H4, which forms the
eukaryotic nucleosome octomer core. Using differential
display of mRNA and rapid amplification of cDNA ends
(RACE), a full-length Histone H3.1 cDNA (CsH3) was
isolated from tea leaves. The open reading frame consisted of
411 nucleotides and deduced amino acid sequence comprised
of 136 amino acid residues. CsH3 shared 79–82% and
98% identity at nucleotide and amino acid sequences,
respectively with Histone H3 isolated from other plant species.
During active-growth period of tea, higher expression
was observed in apical buds that decreased gradually with
increasing age of the leaf. During dormancy season, the
expression of CsH3 was severely down-regulated in all the
leaves studied. CsH3 was found to be down regulated
in response to drought stress and ABA treatment and upregulated
by GA3 treatment. A positive association of CsH3
abundance with active cellular growth suggested its role in
plant growth and development.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/222/1/mol.pdf
Singh, K and Kumar, Sanjay and Ahuja, Paramvir Singh (2009) Differential expression of Histone H3 gene in tea (Camellia sinensis (L.) O. Kuntze) suggests its role in growing tissue. Molecular Biology Reports, 36 (3). pp. 537-542.
 
Relation http://ihbt.csircentral.net/222/