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Ornithogalum: a new host of Cucumber mosaic virus (CMV) from India

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Title Ornithogalum: a new host of
Cucumber mosaic virus
(CMV) from India
 
Creator Verma, N
Singh, L
Singh, A K
Kulshrestha, S
Hallan, Vipin
Raikhy, G
Raja, Ram
Zaidi, A A
 
Subject Plant viruses
 
Description Ornithogalum thyrsoides
(family Hyacinthaceae) is an
ornamental cut flower crop which has a long vase life and
is resistant to extreme temperatures. Plants have been
reported to be infected with
Ornithogalum mosaic virus
(OMV) and
Tobacco necrosis virus
(TNV) (Burger &
Wechmar, 1995).
Ornithogalum plants growing in a floriculture field at
the Institute of Himalayan Bioresource Technology,
Palampur, were found exhibiting severe mosaic on the
leaves. The causal virus was found to be transmitted
mechanically, causing local lesions on
Chenopodium
album
and
C. amaranticolor
, and systemic mosaic on
Nicotiana clevelandii
,
N. glutinosa
,
N. megalosiphon
and
N. tabacum
cv. White Burley. In ELISA, the virus
reacted with
Cucumber mosaic virus
(CMV)-specific
antibodies procured from Agdia (USA). Leaf dip
preparations from virus-infected plants in electron microscopy
revealed the presence of
∼
29 nm isometric
particles. In immunoelectron microscopy (IEM), the virus
was readily trapped and decorated with CMV-specific
antibodies.
Total RNA was isolated from healthy and naturally
infected ornithogalum, as well as from inoculated plants,
using RNAqueous (Ambion, USA) as per the manufacturer’s
instructions. Reverse transcription-polymerase chain
reaction (RT-PCR) was performed using the CPTALL
primer pair (Choi
et al
., 1999). Amplicons of expected
size (
∼
950 bp) were obtained in virus-infected and inoculated
plants. No amplification was observed in healthy
ornithogalum plants. PCR-amplified products were cloned
and sequenced (AJ745092). BLAST searches revealed the
nucleotide and the deduced amino acid sequence showing
97 and 93–95% similarity with the available sequences of
CMV subgroup II, respectively, confirming the association
of CMV with the severe mosaic on ornithogalum.
This is the first definitive report of CMV infecting
ornithogalum.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/231/1/ornithogalum_CMV.pdf
Verma, N and Singh, L and Singh, A K and Kulshrestha, S and Hallan, Vipin and Raikhy, G and Raja, Ram and Zaidi, A A (2005) Ornithogalum: a new host of Cucumber mosaic virus (CMV) from India. Plant Pathology, 54. p. 256.
 
Relation http://ihbt.csircentral.net/231/