Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India
IR@CSIR-IHBT
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Title |
Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India
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Creator |
Hallan, Vipin
Kulshrestha, S Raikhy, G Raja, Ram Zaidi, A A |
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Subject |
Plant Biotechnology
Plant viruses |
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Description |
Iris (Iris x hollandica Hort. cv. Bluemagic)p lants showing mosaic symptomsw ere testedf or the presenceo f Iris mild mosaic polyvirus (IMMV) by host range, enzyme linked immunosorbent assay (ELISA) using antibodies specific for IMMV), reverset ranscriptionp olymerasec hain reaction (RT-PCR),i mmunocapturere verset ranscriptionp olymerasec hain reaction (IC-RT -PCR) using potyvirus group specific primers and antibodies specific to IMMV, immune electron microscopy and cytopathology. Presence of IMMV was detected by ELISA, confirmed by RT-PCR, IC-RT-PCR, IEM, cytopathologya nd sequencingo f the cloned PCR product. From the sequenceo f the virus, specific primers were designed and specific detection of IMMV from iris was standardized |
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Date |
2006
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://ihbt.csircentral.net/242/1/poty.pdf
Hallan, Vipin and Kulshrestha, S and Raikhy, G and Raja, Ram and Zaidi, A A (2006) Occurrenceo f Iris mild mosaicp otyvirus in cultivatedi ris in India. Journal of Biotechnology, 5. pp. 94-98. |
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Relation |
http://ihbt.csircentral.net/242/
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