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Possible role of macrophages induced by an irridoid glycoside (RLJ-NE-299A) in host defense mechanism

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Title Possible role of macrophages induced by an irridoid glycoside (RLJ-NE-299A) in host defense mechanism
 
Creator Sidiq, Tabasum
Khajuria, Anamika
Suden, Pankaj
Sharma, Rohit
Singh, Surjeet
Suri, K.A.
Satti, N.K.
Johri, R.K.
 
Subject Pharmacological Sciences
 
Description In order to explore the possible role of macrophages and other necessary immune competent (T and B) cells in
the modulation of immune responses, an attempt was made to study the immunomodulatory effect of an irridoid glycoside (RLJ-NE-299A) isolated from the roots of Picrorhiza kurroa. Both in vitro and in vivo studies were used to evaluate the effect of RLJ-NE-299A on humoral, cellular, and phagocytic activity of macrophages.The data obtained in the present study showed that RLJ-NE-299A significantly increased sheep red blood cell(SRBC) and induced antibody (IgM and IgG) titer and delayed type hypersensitivity (DTH) reaction in mice.Besides augmenting the humoral and cell-mediated immune response, it induced macrophage phagocytosis
and stimulated cytokine-induced macrophage activation and nitric oxide (NO) production, which resulted in a high degree of protection against Candida albicans and Salmonella typhimurium infections. Flow cytometric
analysis indicated the enhanced expression of co-stimulatory surface molecules CD80 and CD86. The ability of RLJ-NE-299A to upregulate these cell surface antigens involved in antigen presentation may provide an
explanation for the increased T-cell mediated immunity involving macrophages. Taken together this in vitro and in vivo preclinical data suggests that RLJ-NE-299A acts as an effective immunomodulator specifically to improve macrophage function during infections. The effects of this agent on these cells at concentrations relevant to in vivo therapy support its immunopharmacologic application to modify cellular immunity.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/313/1/10.1016j.intimp.2010.10.017.pdf
Sidiq, Tabasum and Khajuria, Anamika and Suden, Pankaj and Sharma, Rohit and Singh, Surjeet and Suri, K.A. and Satti, N.K. and Johri, R.K. (2011) Possible role of macrophages induced by an irridoid glycoside (RLJ-NE-299A) in host defense mechanism. International Immunopharmacology, 11 (1). pp. 128-135. ISSN 15675769
 
Relation http://dx.doi.org/10.1016/j.intimp.2010.10.017
http://iiim.csircentral.net/313/