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Microwave- and ultrasound-assisted extraction of vanillin and its quantification by high-performance liquid chromatography in Vanilla planifolia

IR@CSIR-IHBT

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Title Microwave- and ultrasound-assisted extraction of
vanillin and its quantification by high-performance
liquid chromatography in Vanilla planifolia
 
Creator Sharma, A
Verma, S C
Saxena, N
Chadda, N
Singh, N P
Sinha, Arun K
 
Subject Information and Science Management
 
Description Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and conventional
extraction of vanillin and its quantification by HPLC in pods of Vanilla planifolia
is described. A range of nonpolar to polar solvents were used for the extraction
of vanillin employing MAE, UAE and conventional methods. Various extraction
parameters such as nature of the solvent, solvent volume, time of irradiation, microwave
and ultrasound energy inputs were optimized. HPLC was performed on RP
ODS column (4.6 mm ID6250 mm, 5 lm, Waters), a photodiode array detector
(Waters 2996) using gradient solvent system of ACN and ortho-phosphoric acid in
water (0.001: 99.999 v/v) at 258C. Regression equation revealed a linear relationship
(r 2 A0.9998) between the mass of vanillin injected and the peak areas. The detection
limit (S/N = 3) and limit of quantification (S/N = 10) were 0.65 and 1.2 lg/g, respectively.
Recovery was achieved in the range 98.5–99.6% for vanillin. Maximum yield
of vanilla extract (29.81, 29.068 and 14.31% by conventional extraction, MAE and
UAE, respectively) was found in a mixture of ethanol/water (40 :60 v/v). Dehydrated
ethanolic extract showed the highest amount of vanillin (1.8, 1.25 and 0.99% by
MAE, conventional extraction and UAE, respectively).
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/258/1/sep02.pdf
Sharma, A and Verma, S C and Saxena, N and Chadda, N and Singh, N P and Sinha, Arun K (2006) Microwave- and ultrasound-assisted extraction of vanillin and its quantification by high-performance liquid chromatography in Vanilla planifolia. Journal of Separation Science, 29. pp. 613-619.
 
Relation http://ihbt.csircentral.net/258/