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Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay

IR@CSIR-IIIM

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Title Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay
 
Creator Kumar, Manoj
Khan, Inshad Ali
Verma, Vijashoor
Kalyan, Narender
Qazi, Giulam Nabi
 
Subject Biological Sciences
 
Description A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/328/1/10.1016j.diagmicrobio.2005.05.011.pdf
Kumar, Manoj and Khan, Inshad Ali and Verma, Vijashoor and Kalyan, Narender and Qazi, Giulam Nabi (2005) Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay. Diagnostic Microbiology and Infectious Disease, 53 (2). pp. 121-124. ISSN 07328893
 
Relation http://dx.doi.org/10.1016/j.diagmicrobio.2005.05.011
http://iiim.csircentral.net/328/