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A CsGS is regulated at transcriptional level during developmental stages and nitrogen utilization in Camellia sinensis (L.) O. Kuntze

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Title A CsGS is regulated at transcriptional level during developmental
stages and nitrogen utilization in Camellia sinensis (L.) O. Kuntze
 
Creator Rana, N K
Mohanpuria, P
kumar, Vinay
Yadav , S K
 
Description Glutamine synthetase is a very important
enzyme of ammonium assimilation in plants. Here, we
report on the regulation of a cytosolic glutamine synthetase
(CsGS) from Camellia sinensis (L.) O. Kuntze during
developmental stages and light/dark conditions on the utilization
of nitrate and ammonia. The CsGS expression
levels decreased during dormancy compared to non-dormancy
phase of growth. Different leaf positions present
different ages of the leaf and CsGS expression level was
highest in apical bud (youngest leaf) and lower in mature
fourth leaf, suggesting transcriptional regulation of CsGS
during developmental stages. The CsGS enzyme activity
showed similar trend to that of expression during developmental
stages. The nitrate, ammonium and total amino
acid contents were increased upon exposure to both
N-sources during light and dark conditions. During light
conditions, expression of CsGS in apical bud increased
upon exposure to both N-sources nitrate and ammonium.
While during dark conditions expression was increased
only by ammonium and nitrate had no influence. Exposure
to both N-sources also showed enhancing effect on CsGS
enzyme activity during light. Under dark, ammonium
application increased CsGS enzyme activity and nitrate had
inhibitory effect on the activity. Results suggest the transcriptional
regulation of CsGS on N-utilization during
light/dark conditions.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/269/1/443_2010_A_CsGS.pdf
Rana, N K and Mohanpuria, P and kumar, Vinay and Yadav , S K (2009) A CsGS is regulated at transcriptional level during developmental stages and nitrogen utilization in Camellia sinensis (L.) O. Kuntze. Molecular Biology Reports, 37. pp. 703-710.
 
Relation http://ihbt.csircentral.net/269/