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Computational Structural Analysis and Kinetic Studies of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze

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Title Computational Structural Analysis and Kinetic Studies
of a Cytosolic Glutamine Synthetase from Camellia sinensis
(L.) O. Kuntze
 
Creator Yadav , S K
 
Description Structural analysis of a cytosolic glutamine
synthetase from Camellia sinensis (CsGS) has been conducted
employing computational techniques. This was
conducted to compare its structural aspects with other
known structures of GS. The disordered residues and their
distribution in CsGS are in close comparison to earlier
reported GS. The 3-D structure of CsGS also showed high
degree of similarity with the only known crystal structure
of GS from Zea mays. The Km values observed with
recombinant CsGS for all the three substrates are higher
compared to rice, Arabidopsis, maize and human. This
suggests lower affinity of CsGS for substrates. Further,
kinetic mechanism of CsGS catalysis was investigated
using initial velocity analysis and product inhibition studies.
Initial velocity data eliminate the possibility of pingpong
mechanism and favor the random mechanism of
catalysis. Through product inhibition studies, ADP was
found to be a competitive inhibitor with respect to ATP and
noncompetitive inhibitor versus both glutamate and
ammonium. While, glutamine and inorganic phosphate
were found to be non-competitive inhibitors of ATP, glutamate
and ammonia. Taken together, these observations
are consistent with a random catalysis mechanism for the
CsGS where the binding order of certain substrates is
kinetically preferred by the enzyme.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/272/1/477_2009_Computational.pdf
Yadav , S K (2009) Computational Structural Analysis and Kinetic Studies of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze. The Protein Journal, 28. pp. 428-434.
 
Relation http://ihbt.csircentral.net/272/