Record Details

Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila

IR@CSIR-IIIM

View Archive Info
 
 
Field Value
 
Title Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila
 
Creator Pandey, Anjali
Bani, Sarang
Sangwan, Payarelal
Koul, Surrinder
 
Subject Pharmacological Sciences
 
Description Aim of the study: Labisia pumila has rejuvenating properties that help women to regain the strength and
vigour after giving birth (Burkill, 1935) leading us to speculate that Labisia pumila might also modulate the immune matrix as it is one of the important aspect of healthy state of the living body.
Materials and methods: Studies were carried out for the evaluation of pro-inflammatory cytokines in murine neutrophils followed by expression of T cell-surface markers and corresponding intracellular expression of cytokines related to T helper1 (Th1) and T helper 2 (Th2) activity.
Results: The ethyl acetate fraction (A001/3b) obtained from ethanolic extract of leaves of Labisia pumila at graded doses (in vitro) showed increased expression of TNF-�, IL-6 and IL-12. IL-12 is a strong inducer of Th1 response in activated CD4+ T cells and accordingly T cell studies were carried out (in vivo). These specific studies showed A001/3b to cause marked stimulation of CD4+ T helper cells and related cytokine expression like IL-2 and IFN-gamma (a potent Th1 inducer), thereby, having a predominant Th1 upregulation pathway. This was further validated by suppression of IL-4 and IL-10 expression which are the
Th2 pathway cytokines.
Conclusion: This study showed ethyl acetate fraction (A001/3b) of Labisia pumila to have Th1 upregulating
activity and suggests its possible usefulness as a therapeutic agent in immune compromised patients.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/338/1/10.1016j.jep.2010.08.039.pdf
Pandey, Anjali and Bani, Sarang and Sangwan, Payarelal and Koul, Surrinder (2010) Selective Th1 upregulation by ethyl acetate fraction of Labisia pumila. Journal of Ethnopharmacology, 132 (1). pp. 309-315. ISSN 03788741
 
Relation http://dx.doi.org/10.1016/j.jep.2010.08.039
http://iiim.csircentral.net/338/