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Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze)

IR@CSIR-IHBT

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Title Genomic mapping and testing for quantitative trait loci
in tea (Camellia sinensis (L.) O. Kuntze)
 
Creator Kamunya, S M
Wachira, F N
Pathak, R S
Korir, R
Sharma, V
Kumar, R
Bhardwaj, P
Ahuja, Paramvir Singh
Chalo, R
Sharma, R K
Kumar, R
 
Subject Nutraceuticals
 
Description The tea industry is significant in the economies
of tea-growing countries. Prospects of improving yield of
made tea genomic information were explored using
clones from a cross between clones TRFCA SFS150 and
AHP S15/10. The 42 clones were tested in two distinct
tea-growing regions in Kenya. Bulk segregant analysis
was performed followed by complete genotyping. Out of
260 informative markers, 100 markers that showed 1:1
segregation were used to construct a linkage map. The
map contained 30 (19 maternal and 11 paternal) linkage
groups that spanned 1,411.5 cM with mean interval of
14.1 cM between loci. Based on the map, quantitative
trait loci (QTL) analysis was done on yield data over
2003–2007 across the two sites, Timbilil and Kangaita.
Twenty-three putative QTLs were detected, 16 in five
different linkage groups for Timbilil, two in two groups
for Kangaita, and the rest were associated with unassigned
markers. No QTL was detected at both sites, which
showed strong genotype×site interaction (G×E) but
highly effective within-site heritability (^h2 generally>
0.7). Problems of overestimated and spurious QTL effects
arising from the smallness of the population should be
mitigated by generally high within-site heritability. At
least two unassigned markers associated with yield at
Kangaita over the whole study period, suggesting potential
as candidate markers for site-specific marker-assisted
selections. Implications of the results with respect to
mapping population, G×E, and marker-assisted selection
are discussed.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/279/1/517_2010_Genomic_mapping.pdf
Kamunya, S M and Wachira, F N and Pathak, R S and Korir, R and Sharma, V and Kumar, R and Bhardwaj, P and Ahuja, Paramvir Singh and Chalo, R and Sharma, R K and Kumar, R (2010) Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze). Tree Genetics & Genomes, 6. pp. 915-929.
 
Relation http://ihbt.csircentral.net/279/