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Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice

IR@CSIR-IIIM

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Title Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice
 
Creator Sachin, B.S.
Koul, M.
Zutshi, A.
Singh, S.K.
Tikoo, A.K.
Tikoo, M.K.
Saxena, A.K.
Sharma, S.C.
Johri, R.K.
 
Subject Chemical Sciences
Pharmacological Sciences
 
Description A specific and sensitive high-performance liquid chromatographic (HPLC) method with photodiode-array (PDA) ultraviolet detection was developed for the simultaneous determination of three bioactive constituents of Cedrus deodara namely wikstromol, matairesinol and dibenzylbutyrolactol in mouse plasma. In solid-phase extraction (SPE) these constituents were successfully separated using a C18 column by isocratic elution
using acetonitrile:water containing hexanesulphonic acid, 32:68 (v/v). The flow rate was set at 1 ml/min and detector wavelength at 225 nm. Good linearity (r2 > 0.999) was observed over the studied range of 0.015–5.0 �g/ml for wikstromol and 0.030–5.0 �g/ml for matairesinol and dibenzylbutyrolactol.The CV values of intra-day precision for wikstromol, matairesinol and dibenzylbutyrolactol were in between 1.8–6.9, 1.7–4.9 and 1.6–4.2% and values of inter-day precision were in between 10.4–12.2, 9.7–11 and 10–11.2%, respectively. The extraction recoveries at low to high concentration were greater than 98, 83 and 87% for each analyte, respectively. The LOQ for wikstromol was 0.015 �g/ml and for both matairesinol and dibenzylbutyrolactol it was 0.030 �g/ml. The developed method was used to determine the pharmacokinetics of the three analytes in mice after intraperitoneal administration of CD-3.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/342/1/10.1016j.jchromb.2007.12.015.pdf
Sachin, B.S. and Koul, M. and Zutshi, A. and Singh, S.K. and Tikoo, A.K. and Tikoo, M.K. and Saxena, A.K. and Sharma, S.C. and Johri, R.K. (2008) Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice. Journal of Chromatography B, 862 (1-2). pp. 237-241. ISSN 15700232
 
Relation http://dx.doi.org/10.1016/j.jchromb.2007.12.015
http://iiim.csircentral.net/342/