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Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein

IR@CSIR-IHBT

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Title Molecular variability analyses of Apple chlorotic leaf spot virus
capsid protein
 
Creator Rana, T
Chandel, V
Kumar, Y
Hallan, Vipin
Raja, Ram
Zaidi, A A
 
Subject Plant Biotechnology
Plant viruses
 
Description The complete sequences of the coat protein (CP) gene of 26 isolates of Apple chlorotic leaf spot virus (ACLSV) from
India were determined. The isolates were obtained from various pome (apple, pear and quince) and stone (plum,
peach, apricot, almond and wild Himalayan cherry) fruit trees. Other previously characterized ACLSV isolates and
Trichoviruses were used for comparative analysis. Indian ACLSV isolates among themselves and with isolates from
elsewhere in the world shared 91–100% and 70–98% sequence identities at the amino acid and nucleotide levels,
respectively. The highest degree of variability was observed in the middle portion with 9 amino acid substitutions in
contrast to the N-terminal and C-terminal ends, which were maximally conserved with only 4 amino acid substitutions.
In phylogenetic analysis no reasonable correlation between host species and/or geographic origin of the isolates was
observed. Alignment with capsid protein genes of other Trichoviruses revealed the TaTao ACLSV peach isolate to be
phylogenetically closest to Peach mosaic virus, Apricot pseudo chlorotic leaf spot virus and Cherry mottle leaf virus.
Recombination analysis (RDP3 ver.2.6) done for all the available ACLSV complete CP sequences of the world and
Indian isolates indicate no signifi cant evidence of recombination. However, one recombination event among Indian
ACLSV-CP isolates was detected. To the best of our knowledge, this is the fi rst report of complete CP sequence
variability study from India and also the fi rst evidence of homologous recombination in ACLSV.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/283/1/550_2010_Molecular.pdf
Rana, T and Chandel, V and Kumar, Y and Hallan, Vipin and Raja, Ram and Zaidi, A A (2010) Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. Journal of Biosciences, 35. 605 -615.
 
Relation http://ihbt.csircentral.net/283/