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Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

IR@CSIR-IHBT

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Title Molecular and morphological characterization of somatic hybrids
between Solanum tuberosum L. and S. etuberosum Lindl.
 
Creator Tiwari, J k
Sarkar, D
Pandey, S K
Jai, Gopal
Kumar, R S
 
Subject Plant Biotechnology
 
Description Interspecific potato somatic hybrids between
Solanum tuberosum L. (di)haploid C-13 and 1 endosperm
balance number non-tuberous wild species S. etuberosum
Lindl. were produced by protoplasts electrofusion. The
objective was to transfer virus resistance from this wild
species into the cultivated potatoes. Post-fusion products
were cultured in VKM medium followed by regeneration
of calli in MS13 K medium at 20�C under a 16-h photoperiod,
and regenerants were multiplied on MS medium.
Twenty-one somatic hybrids were confirmed by RAPD,
SSR and cytoplasm (chloroplast/mitochondria) type analysis
possessing species-specific diagnostic bands of corresponding
parents. Tetraploid nature of these somatic
hybrids was determined through flow cytometry analysis.
Somatic hybrids showed intermediate phenotypes (plant,
leaves and floral morphology) to their parents in glasshouse
grown plants. All the somatic hybrids were malefertile.
ELISA assay of somatic hybrids after artificial
inoculation of Potato virus Y (PVY) infection reveals high
PVY resistance.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/286/1/583_2010_Molecular.pdf
Tiwari, J k and Sarkar, D and Pandey, S K and Jai, Gopal and Kumar, R S (2010) Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tissue and Organ Culture, 103 (2). pp. 175-187.
 
Relation http://ihbt.csircentral.net/286/