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Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)

IR@CSIR-IIIM

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Title Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)
 
Creator Yadav, Akhilesh K.
Yadav, Deepti
Shanker, Karuna
Verma, Ram K.
Saxena, Ajit K.
Gupta, Madan M.
 
Subject Chemical Sciences
 
Description An LC method is developed for the quantitation of rhoifolin in Uraria picta, a plant of high usage frequency in all Asian traditional systems of medicine. An isocratic RP-LC method using C18 column, UV detection 265 nm and specificity with PDA and MS is speeding up, reliable
and comprehensive analysis of rhoifolin in U. picta. Good linearity was obtained in the working range (0.02–0.10 mg mL-1), with correlation coefficients >0.99. LOD and LOQ
were 2.33 and 7.69 ng, respectively. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of U. picta extracts as well as Ayurvedic drugs based on Prishniparni.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/376/1/10.1365s10337-009-0963-9.pdf
Yadav, Akhilesh K. and Yadav, Deepti and Shanker, Karuna and Verma, Ram K. and Saxena, Ajit K. and Gupta, Madan M. (2009) Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta). Chromatographia, 69 (7-8). pp. 653-658. ISSN 0009-5893
 
Relation http://dx.doi.org/10.1365/s10337-009-0963-9
http://iiim.csircentral.net/376/