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Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery

IR@CSIR-IIIM

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Title Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery
 
Creator Pal-Bhadra, M.
Leibovitch, B.A.
Gandhi, Sumit
Rao, Madhusudana
Bhadra, Utpal
Birchler, J.A.
Elgin, S.C.R.
 
Subject Biological Sciences
 
Description Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV).Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase.Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://iiim.csircentral.net/377/1/10.1126science.1092653.pdf
Pal-Bhadra, M. and Leibovitch, B.A. and Gandhi, Sumit and Rao, Madhusudana and Bhadra, Utpal and Birchler, J.A. and Elgin, S.C.R. (2004) Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery. Science, 303 (5658). pp. 669-672. ISSN 0036-8075
 
Relation http://dx.doi.org/10.1126/science.1092653
http://iiim.csircentral.net/377/