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Regeneration in Rheum emodi Wall.: A step towards conservation of an endangered medicinal plant species

IR@CSIR-IHBT

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Title Regeneration in Rheum emodi Wall.: A step
towards conservation of an endangered
medicinal plant species
 
Creator Malik, S
Kumar, R
Vats, S K
Bhushan, Shashi
Sharma , Mohit
Ahuja, Paramvir Singh
 
Subject Information and Science Management
 
Description Shoot buds emerged from intact leaves while still attached to plants after 60 days,
without subculturing, on Murashige and Skoog (MS) medium containing 10.0 mM
6-benzylaminopurine and 5.0 mM indole-3-butyric acid. Histological studies
revealed the emergence of shoot buds directly from the leaf surface without an
intervening callus phase. Regenerated plantlets were successfully transferred to the
greenhouse after rooting in plant growth regulator-free MS medium. The survival
percentage of transferred plants was very low. However, pretreatment of the plants
at low temperature (15721C) for 10 days before transfer to greenhouse conditions
increased the survival percentage. The increased survival was supported by an
increased number of vascular bundles, leaf thickness, net rate of photosynthesis
(PN), stomatal conductance (gs) and density (SD). The direct regeneration method
from intact leaves of R. emodi will reduce the cost, time and labor required and
hence can be successfully employed for faster in vitro multiplication and conservation
of this endangered and medicinally important plant species.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/329/1/424_2009_Regeneration.pdf
Malik, S and Kumar, R and Vats, S K and Bhushan, Shashi and Sharma , Mohit and Ahuja, Paramvir Singh (2009) Regeneration in Rheum emodi Wall.: A step towards conservation of an endangered medicinal plant species. Engineering in Life Sciences, 9 (2). pp. 130-134.
 
Relation http://ihbt.csircentral.net/329/