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p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]

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Title p-Coumarate:CoA ligase as a key gene in the yield
of catechins in tea [Camellia sinensis (L.) O. Kuntze]
 
Creator Rahi, A
Singh, A K
Sood, Priyanka
Kumar, Sanjay
Ahuja, Paramvir Singh
 
Subject Nutraceuticals
 
Description Tea is an important crop known for its beverage and antioxidant polyphenols—catechins and its derivatives. Catechins are synthesized through flavonoid (FL) pathway and stored in the vacuole. A metabolic flux for the operation of FL pathway is maintained through the supply of 4-coumaroyl-CoA of phenylpropanoid pathway. 4-Coumaroyl-CoA is synthesized through the catalytic activity of p-coumarate:CoA ligase (4CL) using 4-coumaric acid and acetyl-CoA as the substrates. The present manuscript reports the full-length cDNA cloning of 4CL from tea (Cs4CL accession number DQ194356) and its association with catechin yield. Cs4CL comprised of 2,165 bp with an open reading frame (ORF) of 1,764 nt, starting from 118 to 1,882 encoding 588 amino acids. Altering catechin content through a variety of environmental conditions such as drought stress (DS), abscisic acid (ABA) and gibberellic acid (GA3) treatments, and wounding established a strong positive correlation coefficient between catechins content and the expression of Cs4Cl. In addition, tea clones with high levels of catechins had higher expression of Cs4Cl whereas tea clones with lower catechins exhibited lower expression of this gene. Exposure of tea shoots to 50–100 μM catechins led to down-regulation of the expression of Cs4CL suggesting product-mediated feedback regulation and an important role for the phenylpropanoid pathway in determining catechin yield in tea.
 
Publisher Springer Science
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/336/1/444_2009_p-Coumarate.pdf
Rahi, A and Singh, A K and Sood, Priyanka and Kumar, Sanjay and Ahuja, Paramvir Singh (2009) p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]. Functional and Intergrative Genomics, 9 (2). pp. 271-275. ISSN 1438-793X
 
Relation http://www.springerlink.com/content/a87q03k626754196/fulltext.pdf
http://ihbt.csircentral.net/336/