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Simultaneous densitometric determination of shikonin, acetylshikonin, and b-acetoxyisovalerylshikonin in ultrasonic-assisted extracts of four Arnebia species using reversed-phase thin layer chromatography

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Title Simultaneous densitometric determination of
shikonin, acetylshikonin, and b-acetoxyisovalerylshikonin
in ultrasonic-assisted extracts of four
Arnebia species using reversed-phase thin layer
chromatography
 
Creator Sharma, N
Sharma, U K
Gupta , A P
-------, Devla
Sinha, Arun K
Lal, Brij
Ahuja, Paramvir Singh
 
Subject Information and Science Management
 
Description A simple, precise, and rapid high-performance thin-layer chromatographic (HPTLC)
method for the simultaneous quantification of pharmacologically important naphthoquinone
shikonin (1) together with its derivatives acetylshikonin (2), and b-acetoxyisovalerylshikonin
(3) in four species of genus Arnebia (A. euchroma, A. guttata, A.
benthamii, and A. hispidissima) from the Indian subcontinent has been developed. In
addition, the effect of solvents with varying polarity (hexane, chloroform, ethyl acetate,
and methanol) for the extraction of these compounds was studied. HPTLC was
performed on precoated RP-18 F254S TLC plates. For achieving good separation,
mobile phase consisting of ACN/methanol/5% formic acid in water (40:02:08 v/v/v)
was used. The densitometric determination of shikonin derivatives was carried out
at 520 nm in reflection/absorption mode. The method was validated in terms of linearity,
accuracy, precision, robustness, and specificity. The calibration curves were
linear in the range of 100–600 ng for shikonin and acetylshikonin, and 100–
1800 ng for b-acetoxyisovalerylshikonin. Lower LOD obtained for compounds 1–3
were 18, 15, and 12 ng, respectively, while the LOQ obtained were 60, 45, and 40 ng,
respectively.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/339/1/451_2009_Simultaneous.pdf
Sharma, N and Sharma, U K and Gupta , A P and -------, Devla and Sinha, Arun K and Lal, Brij and Ahuja, Paramvir Singh (2009) Simultaneous densitometric determination of shikonin, acetylshikonin, and b-acetoxyisovalerylshikonin in ultrasonic-assisted extracts of four Arnebia species using reversed-phase thin layer chromatography. Journal of Separation Science, 32. pp. 3239-3245.
 
Relation http://ihbt.csircentral.net/339/