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Identification and cross-species amplification of EST derived SSR markers in different bamboo species

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Title Identification and cross-species amplification of EST derived SSR markers in different bamboo species
 
Creator Sharma, V
Bhardwaj, P
Sharma, R K
Sood, A
Ahuja, Paramvir Singh
 
Subject Nutraceuticals
 
Description The availability of expressed sequence data derived from gene discovery programs became an alternative source of mining simple sequence repeat (SSR) and developing inexpensive genetic markers for the crop improvements. In present study, 10 express sequence tags (EST)-SSR markers were identified from Bambusa oldhamii public sequence data base. Transferability to 25 species of Bambusoideae ranged from 30% to 100%. The number of alleles detected per locus ranged from 2 to 10. All the newly identified SSR markers were found to be moderately to highly polymorphic with an average Polymorphic Information Content (PIC) value of 0.54. As these loci represents transcribed region and recorded high level of cross transferability and reliable amplification across the species, demonstrating the utility of these markers for functional and genetic analyses of bamboo species.
 
Publisher Springer, Van Godewijckstraat 30, 3311 GZ Dordrecht, Netherlands
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/344/1/455_2009_Identification.pdf
Sharma, V and Bhardwaj, P and Sharma, R K and Sood, A and Ahuja, Paramvir Singh (2009) Identification and cross-species amplification of EST derived SSR markers in different bamboo species. Conservation Genetics, 10 (3). pp. 721-724. ISSN 1566-0621
 
Relation http://ihbt.csircentral.net/344/