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Molecular characterization and variability analysis of Apple scar skin viroid in India

IR@CSIR-IHBT

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Title Molecular characterization and variability analysis of Apple scar
skin viroid in India
 
Creator Walia, Y
Kumar, Y
Rana, T
Thakur, P D
Sharma, U
Raja, Ram
Hallan, Vipin
Zaidi, A A
 
Subject Plant sciences
Plant viruses
 
Description Apple scar skin viroid (ASSVd) infection is a
major limitation to apple fruit quality and causes huge
economic losses. In surveys of apple orchards in the
northern Indian state of Himachal Pradesh, fruits with
dappling symptoms were noticed. ASSVd was detected
from these fruits and molecularly characterized. Ten clones
from three isolates were sequenced, of which seven were
new sequence variants of ASSVd. The clones had significant
sequence variability (94–100%) with each other.
Variability was more common in the pathogenic domain of
the viroid genome. Four of the clones were 330 nucleotides
(nt) long, and the other six had an additional nucleotide.
Phylogenetic analysis showed close affinity of the present
isolates with some Chinese and Korean isolates. The study
reports seven new variants of ASSVd and also provides the
first molecular evidence of viroid infection (ASSVd) in
apple in India.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/349/1/467_2009_Molecular.pdf
Walia, Y and Kumar, Y and Rana, T and Thakur, P D and Sharma, U and Raja, Ram and Hallan, Vipin and Zaidi, A A (2009) Molecular characterization and variability analysis of Apple scar skin viroid in India. Journal of General Plant Pathology, 75. pp. 307-311.
 
Relation http://ihbt.csircentral.net/349/