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Radioprotection by a herbal preparation of Hippophae rhamnoides, RH-3, against whole body lethal irradiation in mice

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Title Radioprotection by a herbal preparation of
Hippophae rhamnoides, RH-3, against whole body lethal
irradiation in mice
 
Creator Goel, H C
Prasad, J
Singh, S
Sagar, R K
Kumar, I P
Sinha, Arun K
 
Subject Chemical Engineering
 
Description Purpose: Hippophae rhamnoides L. has been well documented to have anti-oxidative, immunostimulative
and regenerative properties and therefore a herbal preparation of H. rhamnoides coded as
RH-3 was investigated for its radioprotective action.
Materials and methods: RH-3 was administered intraperitonially (i.p.) to mice 30 minutes before
whole body irradiation and whole body survival, spleen Colony forming units (CFU) and haematological
parameters were studied. To investigate free radical scavenging and antioxidant potential,
Fenton reaction, radiation mediated OH radical scavenging and chemically generated superoxide
anions scavenging were studied in vitro while inhibition of lipid peroxidation was studied in
liver homogenate of mice.
Results: A dose of 30 mg/kg body weight of RH-3 rendered 82% survival as compared to no survival
in irradiated control. The endogenous CFU counts in mouse spleen on 10th post-irradiation
day with and without RH-3 demonstrated radioprotective effect. Various hematological parameters
also corroborated the radioprotective effect of RH-3. In a dose dependent manner, RH-3 inhibited
Fenton reaction and radiation mediated generation of hydroxyl radicals in vitro, superoxide
anion mediated Nitroblue tetrazolium (NBT) reduction and FeSO4 mediated lipid peroxidation
in liver.
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/355/1/15_2002_Radioprotection.pdf
Goel, H C and Prasad, J and Singh, S and Sagar, R K and Kumar, I P and Sinha, Arun K (2002) Radioprotection by a herbal preparation of Hippophae rhamnoides, RH-3, against whole body lethal irradiation in mice. Phytomed, 9. pp. 15-25.
 
Relation http://ihbt.csircentral.net/355/