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Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture.

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Title Production of Cucumber mosaic virus-free chrysanthemums by
meristem tip culture.
 
Creator Verma, N
Raja, Ram
Hallan, Vipin
Kumar, K
Zaidi, A A
 
Subject Plant sciences
Plant viruses
 
Description Chrysanthemum cv. Regol Time was found infected with a viral disease showing characteristic chlorotic spots on leaves and
stunting of plants. The virus was identified as Cucumber mosaic cucumovirus (CMV) on the basis of mechanicalinocul ation on
Chenopodium amaranticolor and Cucumis sativus, DAS-ELISA and RT-PCR. CMV was eliminated from chrysanthemum plants
using meristem tip culture. MS medium amended with BAP (2 mg/l) and IBA (0.05 mg/l) was used for shoot proliferation and IBA
(0.05 mg/l) for rooting of the plants. Virus-free plants (84%) were obtained from the optimum size (0.3 mm) of meristem tips (as
indexed by DAS-ELISA). Of these, 72% plants were found negative for CMV as indexed by RT-PCR. Virus indexing by RT-PCR
was found to be a reliable method.
r 2003 Elsevier Ltd. All rights reserved.
 
Publisher Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England .
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/379/1/111_A_2004_Production.pdf
Verma, N and Raja, Ram and Hallan, Vipin and Kumar, K and Zaidi, A A (2004) Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture. Crop Protection, 23 (5). pp. 469-473. ISSN 0261-2194
 
Relation http://ihbt.csircentral.net/379/