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A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography

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Title A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography
 
Creator Mishra, Nidhi
Gupta , A P
Singh , Bikram
Kaul , V K
Ahuja, Paramvir Singh
 
Subject Natural Product Chemistry
 
Description A sensitive, selective, and precise RP-HPTLC method has been developed for the quantification of podophyllotoxin in Podophyllum hexandrum, collected from different locations of the western Himalaya of India, ranging from 3250 to 4100 meters above the mean sea level. The assay employed RP-18 F-254 TLC plates (chromatoplates) as the stationary phase. The solvent system consisted of acetonitrile-water (4: 6, v/v) and gave better resolution, with well separated, compact spots for podophyllotoxin (Rf value 0.41 +/- 0.02). Densitometric analysis was carried out in the absorption remission mode at 217 nm.
 
Publisher Taylor & Francis, Inc., 325 Chestnut Street, Suite 800, Philadelphia, PA 19106
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/400/1/145_2005_A_Rapid.pdf
Mishra, Nidhi and Gupta , A P and Singh , Bikram and Kaul , V K and Ahuja, Paramvir Singh (2005) A rapid determination of podophyllotoxin in podophyllum hexandrum by reverse phase high performance thin layer chromatography. Journal of Liquid Chromatography & Related Technologies, 28 (5). pp. 677-691. ISSN 1082-6076
 
Relation http://ihbt.csircentral.net/400/