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First finding of Freesia mosaic virus infecting freesia in India

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Title First finding of
Freesia mosaic virus
infecting freesia in India
 
Creator Kumar, Y
Hallan, Vipin
Zaidi, A A
 
Subject Plant sciences
Plant viruses
 
Description Freesias are popular garden plants in the family Iridaceae.
Freesia
spp.
have been reported to be infected by various viruses:
Bean yellow mosaic
virus
,
Freesia mosaic virus
,
Cucumber mosaic virus
,
Tobacco rattle virus
(Brunt, 1995) and ophioviruses (Vaira
et al
., 2006). In April 2007, leaf
samples showing chlorotic streaks were collected from Kangra region of
Northern India. These samples were tested for the presence of potyviruses
using an indirect ELISA kit (Agdia) as per the manufacturer’s instructions.
A positive result was obtained from one of the ten collected samples. Total
RNA was extracted from this positive leaf sample using RNAqueous®
RNA isolation kit (Ambion). RT-PCR was performed with a degenerate
potyvirus primer pair (P9502 & CPUP), which amplifies the partial coat
protein (CP) gene and 3
′
-UTR region of the viral genome (Van der Vlugt
et al
., 1999). An amplicon of ~650 bp was obtained, which was cloned into
pGEM®-T Easy vector (Promega) and sequenced. Analysis showed that
the sequence shared 98% identity with the only sequence of the tentativelynamed
Spiranthes mosaic virus 2 (SpMV2; GenBank Accession No.
AY685219), reported from
Spiranthes cernua
in USA (Guaragna
et al
.,
2006). In order to sequence the full CP gene, primers were designed using
the available SpMV2 sequence. The complete CP gene and 3
′
-UTR was
amplified, cloned and sequenced. A sequence of 1026 bp was submitted to
the EMBL Database (Accession No. AM748701). This sequence showed
97% identity with a partial sequence (429 bp) of
Freesia mosaic virus
(FreMV; EF203688) obtained from a Dutch
Freesia
isolate, this short
sequence is the only one available for FreMV. The Indian isolate showed
less than 86% homology at the amino acid level with any other potyvirus.
A host range study using the Indian isolate was carried out using
Freesia
and
Spiranthes
spp. The isolate could not be transmitted to the orchid; but
it did infect
Freesia
, showing mild chlorotic symptoms, typical of FreMV
infection. Based on the biological and sequence data, the present isolate
has been identified as FreMV. This study also supports the case that
SpMV2 is in fact an isolate/strain of FreMV, rather than being a distinct
virus. This is the first report of FreMV in India.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/497/1/283_2009_2-12-2011_First.pdf
Kumar, Y and Hallan, Vipin and Zaidi, A A (2009) First finding of Freesia mosaic virus infecting freesia in India. Plant Pathology, 58. p. 404.
 
Relation http://ihbt.csircentral.net/497/