Record Details

Genetic Relatedness of Trichoderma Isolates Antagonistic against Fusarium oxysporum f.sp. dianthi Inflicting Carnation Wilt

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title Genetic Relatedness of Trichoderma Isolates Antagonistic
against Fusarium oxysporum f.sp. dianthi Inflicting
Carnation Wilt
 
Creator Shanmugam, V
Sharma, V
-------,, Ananthapdmanaban
 
Subject Microbiology
 
Description Twenty-eight isolates of Trichoderma belonging to four different species were screened in
vitro for their antagonistic ability against Fusarium oxysporum f.sp. dianthi causing carnation wilt. Three different
levels of antagonism observed in dual plate assay were further confirmed by cell-free culture filtrate
experiments. Isolates showing class I level of antagonism produced maximum lytic enzymes, chitinases and
�-1,3-glucanases. Genetic variability of 25 selected isolates was assessed by random amplified polymorphic
DNA technique and the amplified products were correlated for their level of antagonism. Unweighed pairgroup
method with arithmetical averages cluster analysis revealed prominent inter- and intraspecific genetic
variation among the isolates. Based on their genetic relationship, the isolates were mainly distributed into
3 major groups representing T. atroviride, T. pseudokoningii and T. harzianum, with 20–35 % interspecific
dissimilarity. However, the polymorphism shown by the isolates did not correlate to their level of antagonism.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/503/1/292_2008_2-12-2011_Genetic.pdf
Shanmugam, V and Sharma, V and -------,, Ananthapdmanaban (2008) Genetic Relatedness of Trichoderma Isolates Antagonistic against Fusarium oxysporum f.sp. dianthi Inflicting Carnation Wilt. Folia Microbiologia, 53 (2). pp. 130-138.
 
Relation http://ihbt.csircentral.net/503/