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Microwave-Assisted Efficient Extraction of Different Parts of Hippophae rhamnoides for the Comparative Evaluation of Antioxidant Activity and Quantification of Its Phenolic Constituents by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)†

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Title Microwave-Assisted Efficient Extraction of Different
Parts of Hippophae rhamnoides for the Comparative
Evaluation of Antioxidant Activity and Quantification
of Its Phenolic Constituents by Reverse-Phase
High-Performance Liquid Chromatography
(RP-HPLC)†
 
Creator Sharma, U K
Sharma, K
Sharma , N
Sharma, A
Singh, H P
Sinha, Arun K
 
Subject Pest and Pesticides
 
Description The outcome of different extraction procedures (microwave, ultrasound, Soxhlet, and maceration)
on the antioxidant activity of seeds, leaves, pulp, and fruits of Hippophae rhamnoides (sea buckthorn
or SBT) was investigated by two different bioassays: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) diammonium salt (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays.
The SBT extracts were found to possess strong antioxidant activity measured in terms of TEAC
(2.03–182.13 and 6.97–282.75 mg/g) with ABTS and DPPH assays, respectively. In general, the
antioxidant capacity of microwave-assisted extracts was found to be significantly higher than those
obtained by ultrasound-assisted extraction (UAE) and maceration while being slightly higher than
Soxhlet extracts. Further, microwave extracts of seeds were found to possess maximum antioxidant
capacity followed by leaves, fruits, and pulp. Also, the chemical composition of extracts, studied in
terms of the total phenolic content, was found to be in the range of 1.9–23.5 mg/g Gallic acid equivalent
(GAE), which indicates a strong correlation between antioxidant activity and phenolic content present
in the SBT. In addition, some of its bioactive phenolic constituents, such as rutin (1), quercetin-3-
O-galactoside (2), quercetin (3), myricetin (4), kaempferol (5), and isorhamnetin (6), were also
quantified in different extracts by reverse-phase high-performance liquid chromatography (RP-HPLC).
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/514/1/374_2008_2-12-2011.pdf
Sharma, U K and Sharma, K and Sharma , N and Sharma, A and Singh, H P and Sinha, Arun K (2008) Microwave-Assisted Efficient Extraction of Different Parts of Hippophae rhamnoides for the Comparative Evaluation of Antioxidant Activity and Quantification of Its Phenolic Constituents by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)†. Journal of Agriculture and Food Chemistry, 56. pp. 374-379.
 
Relation http://ihbt.csircentral.net/514/