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RP-HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*

IR@CSIR-IHBT

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Title RP-HPTLC densitometric determination
and validation of vanillin and related phenolic
compounds in accelerated solvent extract
of Vanilla planifolia*
 
Creator Sharma, U K
Sharma, N
Gupta , A P
Kumar, Vinod
Sinha, Arun K
 
Subject Natural Product Chemistry
 
Description A simple, fast and sensitive RP-HPTLC method is developed for simultaneous quantitative
determination of vanillin and related phenolic compounds in ethanolic
extracts of Vanilla planifolia pods. In addition to this, the applicability of accelerated
solvent extraction (ASE) as an alternative to microwave-assisted extraction (MAE),
ultrasound-assisted extraction (UAE) and Soxhlet extraction was also explored for
the rapid extraction of phenolic compounds in vanilla pods. Good separation was
achieved on aluminium plates precoated with silica gel RP-18 F254S in the mobile
phase of methanol/water/isopropanol/acetic acid (30:65:2:3, by volume). The method
showed good linearity, high precision and good recovery of compounds of interest.
ASE showed good extraction efficiency in less time as compared to other techniques
for all the phenolic compounds. The present method would be useful for analytical
research and for routine analysis of vanilla extracts for their quality control.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/519/1/375_2007_2-12-2011.pdf
Sharma, U K and Sharma, N and Gupta , A P and Kumar, Vinod and Sinha, Arun K (2007) RP-HPTLC densitometric determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia*. Journal of Separation Science , 30. pp. 3174-3180.
 
Relation http://ihbt.csircentral.net/519/