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Gradient HPLC of antibiotics in urine, ground water, chicken muscle, hospital wastewater, and pharmaceutical samples using C-18 and RP-amide columns

IR@CSIR-IHBT

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Title Gradient HPLC of antibiotics in urine, ground water,
chicken muscle, hospital wastewater, and
pharmaceutical samples using C-18 and RP-amide
columns
 
Creator Kumar, A
Malik, A K
Tewary, D K
Singh , B
 
Subject Information and Science Management
 
Description A simple and highly sensitive high pressure liquid chromatographic (HPLC-UV)
method has been developed for the determination of ofloxacin, lomefloxacin, cinoxacin,
and nalidixic acid, in mobile phase citrate buffer (0.001 M) of pH 4.5 prepared
in water (X), methanol (Y), and ACN (Z) using gradient at a flow rate of 1.0 mL/min
by direct UV absorbance detection at k = 280 nm. Separation of analytes was studied
on the C-18 and RP-amide columns and best results were observed on the RP-amide
column with LODs (3.36S/m) 0.89, 0.55, 0.67, and 1.41 ng/mL for ofloxacin, lomefloxacin,
cinoxacin, and nalidixic acid, respectively, and better RSD than the C-18
column. The recovery of Fluoroquinolones (FQs) in urine, ground water, hospital
wastewater, and chicken muscle using this method is more than 90%. The method
was successfully applied to the analysis of ofloxacin, lomefloxacin, cinoxacin, and
nalidixic acid in urine, ground water, pharmaceutical dosage forms, hospital wastewater,
and chicken muscle.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/528/1/386_2008_2-12-2011.pdf
Kumar, A and Malik, A K and Tewary, D K and Singh , B (2008) Gradient HPLC of antibiotics in urine, ground water, chicken muscle, hospital wastewater, and pharmaceutical samples using C-18 and RP-amide columns. Journal of Separation of Science, 31 (2). pp. 294-300.
 
Relation http://ihbt.csircentral.net/528/