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Biological management of vascular wilt of tomato caused by Fusarium oxysporum f.sp. lycospersici by plant growth-promoting rhizobacterial mixture

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Title Biological management of vascular wilt of tomato caused by
Fusarium oxysporum f.sp. lycospersici by plant growth-promoting
rhizobacterial mixture
 
Creator Shanmugam , V
Kanoujia, N
 
Subject Microbiology
 
Description Two plant growth promoting rhizobacterial (PGPR) strains, GIBC-Jamog (Bacillus subtilis) and TEPF-Sungal
(Burkholderia cepacia) and PGPR strain mixtures, S2BC-1 (B. subtilis)+GIBC-Jamog (B. subtilis) and S2BC-2
(Bacillus atrophaeus)+TEPF-Sungal (Burkholderia cepacia) which inhibited the mycelial growth of vascular
wilt fungus Fusarium oxysporum f.sp. lycopersici were selected for assessing vascular wilt control in tomato
by localized and induced systemic resistance (ISR) experiments in the greenhouse. Seed bacterization and
soil application of S2BC-1+GIBC-Jamog challenge-inoculated with F. oxysporum f.sp. lycopersici resulted in
significantly lower incidence (54.9% and 53.8% reduction) relative to the non-bacterized pathogen control
in localized and split-root experiments, respectively. In localized studies, the lower disease incidence coincided
with increases in the plant vigor index and fresh weight of 44.1% and 183.4%, respectively, relative to
the pathogen control. Analysis of root samples in localized and split-root experiments also indicated
increased induction of chitinase (2.2- and 2.6-fold, respectively) and b-1,3-glucanase (1.4- and 1.1-fold,
respectively). In native gel activity assay, S2BC-1+GIBC-Jamog with challenge-inoculation, expressed high
intensity peroxidase isoforms in localized and ISR experiments. It is presumed that the induced chitinase,
b-1,3-glucanase and peroxidase in localized and split-root experiments may be involved in the reduction
of vascular wilt development in tomato. Reduced disease severity coupled with enhanced enzyme production
elicited by S2BC-1+GIBC-Jamog in localized and split-root experiments indicate that its mode of
action for vascular wilt suppression in tomato is through both direct biocontrol and ISR.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/617/1/554_2011_9-12-2011.pdf
Shanmugam , V and Kanoujia, N (2011) Biological management of vascular wilt of tomato caused by Fusarium oxysporum f.sp. lycospersici by plant growth-promoting rhizobacterial mixture. Bio Control, 57. pp. 85-93.
 
Relation http://ihbt.csircentral.net/617/