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Bacterial diversity and soil enzyme activity in diseased and disease free apple rhizosphere soils

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Title Bacterial diversity and soil enzyme activity in diseased
and disease free apple rhizosphere soils
 
Creator Shanmugam, V
Verma, R
S, Rajkumar
Naruka , D S
 
Subject Plant sciences
Microbiology
 
Description A culture-independent survey of the bacterial
diversity in rhizosphere soils of diseased (scab) and diseasefree
apple trees was conducted to assess the role of bacteria
in disease suppression. Community DNA was extracted
from soil samples and amplified by PCR using primers
specific for bacterial 16S rRNA gene sequences. Clone
libraries were constructed with the PCR products and
analysed based on amplified rDNA restriction analysis
(ARDRA) patterns. The phylotypes and their frequency
distribution in both libraries indicated that the phylotype
did not represent a single group. Rarefaction curve, and
Shannon and Simpson diversity indices exhibited insignificant
diversity differences between the samples in terms of
bacterial community composition, whereas more chitinase
and β-1,3 glucanase activities were recorded in samples
from disease-free trees than from diseased trees. Based on
the operational taxonomic units identified in ARDRA, 80
representative clones were selected from the libraries and
partially sequenced. Sequence similarity searches with the
resulting sequences identified the dominance of uncultured
bacteria to the extent of 70% and 72.5% in disease free and
diseased rhizospheres, respectively. The foregoing studies
conclude a possible role for enhanced microbial activity in
terms of enzyme production in tree (apple) health, although
no distinct partitioning of composition or significant
diversity of bacterial communities inhabiting diseased and
disease-free rhizosphere soils was observed at any given
time.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/619/1/557_2011_9-12-2011.pdf
Shanmugam, V and Verma, R and S, Rajkumar and Naruka , D S (2011) Bacterial diversity and soil enzyme activity in diseased and disease free apple rhizosphere soils. Annals of Microbiology, 61. pp. 767-772.
 
Relation http://ihbt.csircentral.net/619/