Record Details

Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64

IR@CSIR-IHBT

View Archive Info
 
 
Field Value
 
Title Chlorpyrifos degradation by the cyanobacterium
Synechocystis sp. strain PUPCCC 64
 
Creator Singh, D P
Khattar, J I S
Nadda, J
Singh , Y
Grag, A
Kaur, N
Gulati, Ashu
 
Subject Synthetic Chemistry
 
Description Background, aim, and scope Indiscriminate use of insecticides
leads to environmental problems and poses a great
threat to beneficial microorganisms. The aim of the present
work was to study chlorpyrifos degradation by a rice field
cyanobacterium Synechocystis sp. strain PUPCCC 64 so
that the organism is able to reduce insecticide pollution
in situ.
Material and methods The unicellular cyanobacterium isolated
and purified from a rice field was identified by partial
16S rRNA gene sequence as Synechocystis sp. strain
PUPCCC 64. Tolerance limit of the organism was determined
by studying its growth in graded concentrations (2.5–
20 mg/L) of chlorpyrifos. Chlorpyrifos removal was studied
by its depletion from the insecticide supplemented growth
medium, and its biodegradation products were identified in
the cell extract, biomass wash, and growth medium.
Results and discussion The organism tolerated chlorpyrifos
up to 15 mg/L.Major fraction of chlorpyrifos was removed by
the organism during the first day followed by slow uptake.
Biomass, pH, and temperature influenced the insecticide
removal and the organism exhibited maximum chlorpyrifos
removal at 100 mg protein/L biomass, pH 7.0, and 30°C. The
cyanobacteriummetabolized chlorpyrifos producing a number
of degradation products as evidenced by GC-MS chromatogram.
One of the degradation products was identified as 3,5,6-
trichloro-2-pyridinol.
Conclusion and recommendations Present study reports the
biodegradation of chlorpyrifos by Synechocystis sp. Biodegradation
of the insecticide by the cyanobacterium is
significant as it can be biologically removed from the
environment. The cyanobacterium may be used for bioremediation
of chlorpyrifos-contaminated soils.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/622/1/570_2011_9-12-2011.pdf
Singh, D P and Khattar, J I S and Nadda, J and Singh , Y and Grag, A and Kaur, N and Gulati, Ashu (2011) Chlorpyrifos degradation by the cyanobacterium Synechocystis sp. strain PUPCCC 64. Environmental Science and Pollution Research , 18. pp. 1351-1359.
 
Relation http://ihbt.csircentral.net/622/