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Problems and possibilities of monocot transformation

IR@CSIR-IHBT

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Title Problems and possibilities of monocot transformation
 
Creator Sood, Priyanka
Bhattacharya, Amita
Sood, A
 
Subject Plant Biotechnology
 
Description Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite
wound responses and low cell competence. Despite these limitations, the biolistic and Agrobacterium methods have
been successfully used to produce several transgenic monocots by adjusting the parameters that govern efficient
delivery and integration of transgene(s) into plant genome. It is now possible to transform even difficult monocots using
tailor-made gene constructs and promoters, suitable A. tumefaciens strains and a proper understanding of the entire
process. This success has been reviewed in the present article and a special emphasis was laid on the measures that were
taken in overcoming the difficulties that arise due to the differential responses of monocots and dicots. This information
is necessary for biotechnological improvement of still newer monocotyledonous plants that have been hitherto difficult
to transform.
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/624/1/581_2011_9-12-2011.pdf
Sood, Priyanka and Bhattacharya, Amita and Sood, A (2011) Problems and possibilities of monocot transformation. Biologia Plantarum, 55 (1). pp. 1-15.
 
Relation http://ihbt.csircentral.net/624/