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Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro

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Title Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro
 
Creator Godbole, S
Sood, A
Thakur, R
Sharma, Mohit
Ahuja, Paramvir Singh
 
Subject Plant Biotechnology
 
Description New sprouts from the nodal segments of the field-selected, mature, elite bamboos were used for the induction of embryogenic callus. Murashige and Skoog medium with BA and 2,4-D (1.0 mg/l each) was essential for culture establishment and callusing. Subsequently, elimination of 2,4-D and a corresponding increase in BA concentration induced embryogenesis. Upon transfer to sucrose-enriched medium without PGRs, these embryos matured and germinated into plantlets within 21 days, with a conversion rate of about 80%. The protocol thus established showed a low frequency of albinos when embryos were matured on a high sucrose (8%) containing medium under diffuse light conditions (5 mumol m(-2) S-1). Rhizome formation took place upon prolonged culturing in the same medium and served as excellent propagules for multiplication. The plantlets were hardened and up to 78% survival rate was achieved in soil under greenhouse conditions.
 
Date 2002
 
Type Article
PeerReviewed
 
Identifier Godbole, S and Sood, A and Thakur, R and Sharma, Mohit and Ahuja, Paramvir Singh (2002) Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Current Science, 83. pp. 885-9.
 
Relation http://ihbt.csircentral.net/631/