Record Details


Field	Value

Title	Induction of synchronous secondary somatic embryogenesis in Camellia sinensis (L.) O. Kuntze

Creator	Mondal, T K Bhattacharya, Amita Ahuja, Paramvir Singh

Subject	Plant Biotechnology

Description	Nutritional effect of nitrate salts of potassium and ammonium, together with different concentrations of sulphate salts of aluminium, potassium, magnesium, and ammonium on secondary somatic embryogenesis, wereinvestigated. Nitrate salts of potassium (9.39 mmol/L) and ammonium (10.31 mmol/L) with only 1.5 mmol/L potassium sulphate produced maximum number of synchronous secondary embryos (i.e. 20-25 secondary embryos per primary embryo in 91.6 percnt; responsive explants). Of the different factorial combinations of glutamine, BAP, and IBA tested, maximum number of synchronous secondary embryos developed on a medium supplemented with 8.88 μmol/L BAP, 0.98 μmol/L IBA and 10 mmol/L glutamine. Synchronous and normal development of secondary embryos could thus be obtained when optimal concentrations of PGRs, glutamine, nitrates, and salts of potassium sulphate were combined together. Germination of the embryos (up to 52 percnt;) was acheived only when sulphate salts of potassium were removed from the medium.

Date	2001

Type	Article PeerReviewed

Identifier	Mondal, T K and Bhattacharya, Amita and Ahuja, Paramvir Singh (2001) Induction of synchronous secondary somatic embryogenesis in Camellia sinensis (L.) O. Kuntze. J.Pl.Physiol., 158. pp. 945-51.

Relation	http://ihbt.csircentral.net/634/

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