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Validated high-performance thin-layer chromatography method for steviol glycosides in Stevia rebaudiana.

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Title Validated high-performance thin-layer chromatography method for steviol glycosides in Stevia rebaudiana.
 
Creator Jaitak, V
Gupta , A P
Kaul , V K
Ahuja, Paramvir Singh
 
Subject Natural Product Chemistry
Bioinformatics
 
Description A high-performance thin-layer chromatographic (HPTLC) method was developed and validated as per ICH (International Conferences on Harmonization) guidelines for simultaneous quantification of three steviol glycosides, i.e. steviolbioside, stevioside and rebaudioside-A in Stevia rebaudiana leaves. For achieving good separation, mobile phase of ethyl acetate–ethanol–water (80:20:12, v/v/v) on pre-coated silica gel 60 F254 HPTLC plates were used. The densitometric quantification of steviol glycosides was carried out at λ = 510 nm in reflection–absorption mode after spraying with acetic anhydride:sulphuric acid:ethanol reagent. The calibration curves were linear in the range of 160–960 ng/spot for steviolbioside, 1–6 μg/spot for stevioside and 0.5–3 μg/spot for rebaudioside-A with good correlation coefficients (0.998–0.999). The method was found to be reproducible for quantitative analysis of steviol glycosides in S. rebaudiana leaves collected from ten different locations and will serve as a quality control indicator to monitor the commercial production of stevioside and its allied molecules during different stages of its processing.
 
Date 2008
 
Type Article
PeerReviewed
 
Identifier Jaitak, V and Gupta , A P and Kaul , V K and Ahuja, Paramvir Singh (2008) Validated high-performance thin-layer chromatography method for steviol glycosides in Stevia rebaudiana. Journal of Pharmaceutical and Biomedical Analysis, 47. pp. 790-794.
 
Relation http://ihbt.csircentral.net/661/