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Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze)

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Title Purification and partial characterization of a low temperature
responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze)
 
Creator Vyas, D
Kumar, Sanjay
 
Subject Nutraceuticals
 
Description The manganese containing superoxide dismutase (Mn-SOD) was purified from a tea clone, TEENALI, which showed the lowest
period of winter dormancy. Protein was purified using leaves of tea by ammonium sulfate precipitation, followed by column chromatography
using DEAE–cellulose, and silica-based size exclusion chromatography on HPLC system. Upto 51-fold purification
and a specific activity of 56.66 U/mg of protein was achieved, which yielded a single band upon denaturing PAGE. The enzyme
had a native molecular weight of about 169 kDa, whereas a monomer with molecular weight of 43 kDa was found on SDS–PAGE
suggesting it to be homotetramer. The purified enzyme had pH optima of 8.0. It exhibited a wide temperature range for its activity
with optima at 0 degree C suggesting its role in low temperature tolerance. The manuscript presents purification and characterization of
high molecular weight Mn-SOD from tea and discusses its implication in tolerance of low temperature stress.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/670/1/164_12-12-2011.pdf
Vyas, D and Kumar, Sanjay (2005) Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis (L.) O. Kuntze). Biochemical and Biophysical Research Commuications, 329. pp. 831-838.
 
Relation http://ihbt.csircentral.net/670/