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DEVELOPMENT OF DIAGNOSTIC KIT AGAINST THE RECOMBINANT COAT PROTEIN OF PRUNUS NECROTIC RINGSPOT VIRUS.

IR@CSIR-IHBT

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Title DEVELOPMENT OF DIAGNOSTIC KIT AGAINST THE RECOMBINANT COAT PROTEIN OF PRUNUS NECROTIC RINGSPOT VIRUS.
 
Creator Kulshrestha, S
Hallan, Vipin
Raikhy, G
Zaidi, A A
 
Subject Plant sciences
Plant viruses
 
Description 1. Primers useful for detection of Prunus necrotic ringspot virus (PNRSV) in plants, comprising the following sequence: Sequence ID 1: upstream primer AACTGCAGATGGTTTGCCGAATTTGCAA Sequence ID 2: downstream primer GCTCTAGACTAGATCTCAAGCAGGTC 2. A method for detection of Prunus necrotic ringspot virus (PNRSV) in plants, wherein the said method comprising the steps of: b) providing a purified coat protein of PNRSV by using designed primers of Sequence ID 1: upstream primer AACTGCAGATGGTTTGCCGAATTTGCAA Sequence ID 2: downstream primer GCTCTAGACTAGATCTCAAGCAGGTC; b) preparing polyclonal antibodies against PNRSV coat protein obtained from step (a); c) performing direct antibody sandwich enzyme linked immunosorbent assay (DAS ELISA) for detection of PNRSV.
 
Date 2010
 
Type Article
PeerReviewed
 
Identifier Kulshrestha, S and Hallan, Vipin and Raikhy, G and Zaidi, A A (2010) DEVELOPMENT OF DIAGNOSTIC KIT AGAINST THE RECOMBINANT COAT PROTEIN OF PRUNUS NECROTIC RINGSPOT VIRUS. Journal of Prunus necrotic ringspot virus.
 
Relation http://ihbt.csircentral.net/737/