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Occurrence of Chilli veinal mottle virus in Himalayan butterfly bush ( Buddleja crispa )

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Title Occurrence of
Chilli veinal mottle virus
in Himalayan butterfly bush
(
Buddleja crispa
)
 
Creator Mehra, A
Hallan, Vipin
Lal, Brij
Zaidi, A A
 
Subject Plant sciences
Plant viruses
 
Description Buddleja crispa
, commonly known as Himalayan butterfly bush, grows in
open rocky places. It is a deciduous or semi-evergreen, ornamental, perennial
shrub with fuzzy white leaves on long, arching stems, bearing tiny
pink, violet or purple flowers. The flowers are often fragrant, attractive
and irresistible to butterflies, bees and other nectar-feeding birds and
insects.
Buddleja davidii
, an allied species of
B. crispa
, has been reported
to be a host of
Tomato ringspot virus
(Hughes & Scott, 2003),
Alfalfa
mosaic virus
(Walter
et al
., 1985) and
Cucumber mosaic virus
(Perkins,
1991).
In the course of a study to assess virus incidence in natural plant
resources of high-altitude areas in the western Himalayan region of India,
some leaves of
B. crispa
with mild chlorosis and mosaic symptoms were
collected from the Kinnaur area (altitude 2500 m asl) of Himachal
Pradesh during 2003. The infected leaves were tested by ELISA for the
presence of
Tomato ringspot virus
(antibody kit supplied by DSMZ,
Braunschweig, Germany),
Cucumber mosaic virus
and potyviruses (antibodies
from Agdia, Elkhart, USA). A positive result was obtained with the
potyvirus group-specific antibodies. To confirm the identity of the virus
detected, leaf samples were tested using a universal potyvirus primer pair
(P9502 and CPUP), which amplify part of the coat-protein gene and 3
′
-
UTR of the viral genome (Van der Vlugt
et al
., 1999). An amplification
product of 800 bp was obtained after RT–PCR. The DNA product was
cloned, sequenced and submitted to the EMBL Database (accession
number AJ889836). The sequence showed 95% homology to Chilli
vein-banding mottle virus, a strain of
Chilli veinal mottle virus
reported
from Japan (GenBank accession number AB012221). This is a new report
of this potyvirus occurring on
B. crispa
growing in nature, therefore this
plant should be taken into account as a potential reservoir of
Chilli veinal
mottle virus
infecting chilli crops.
 
Date 2006
 
Type Article
PeerReviewed
 
Identifier Mehra, A and Hallan, Vipin and Lal, Brij and Zaidi, A A (2006) Occurrence of Chilli veinal mottle virus in Himalayan butterfly bush ( Buddleja crispa ). Plant Pathology, 55 (2). p. 284.
 
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