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Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron.

IR@CSIR-IHBT

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Title Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron.
 
Creator Kumar , Anil
Sood, A
Palni T, Uma
Gupta , A K
Palni, LMS
 
Subject Plant sciences
Plant Biotechnology
 
Description Summery
An efficient protocol for micropropagation of Rosa damascena Mill.has been established using single node segments from mature bushes. The effect of thidiazuron has been compared with that of N6-benzyladenine(BA)on in vitro shoot proliferation.The cultures initiated on medium supplemented with thidiazuron(TDZ)and/or cultured continuously on TDZ containing medium for 32-48 weeks exhibited considerably more shoot proliferation and growth during subsequent culture on a medium containing BA. Shoots induced on TDZ containing medium and then sub-cultured(8-12 times)on medium containing BA,attained the capacity to grow and proliferate on a medium free from plant growth regulators(PGR). Microshoots from TDZ induced cultures could be rooted easily on indole-3-butyric acid(IBA) supplemented medium. A short treatment with IBA(100uM;12h)was found to be very effective for root induction. The rooted plants were successfully transferred to pots after 15 d of hardening in a mist chamber with about 70% survival.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/895/1/1_Anil_sood.pdf
Kumar , Anil and Sood, A and Palni T, Uma and Gupta , A K and Palni, LMS (2001) Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron. Journal of Horticultural Science & Biotechnology, 76 (1). pp. 30-34.
 
Relation http://ihbt.csircentral.net/895/