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In vitro production of Prunus necrotic ringspot virus-free begonias through chemo- and thermotherapy

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Title In vitro production of Prunus necrotic ringspot
virus-free begonias through chemo- and
thermotherapy
 
Creator Verma, N
Raja, Ram
Zaidi , A A
 
Subject Plant sciences
Plant viruses
 
Description Abstract
Prunus necrotic ringspot virus (PNRSV)-free Begonia spp. plants were raised from petioles of
virus-infected plants using in vitro techniques. The petioles were grown onMSmedium supplemented
with 0.2 mg/l NAA and 0.2 mg/l BAP (pH 5.8). For rooting, half-strength MS medium without any
plant growth regulators was used. On rooting medium, shoots were subjected to chemotherapy (virazole,
2-thiouracil or 6-azauracil) and thermotherapy (38 ◦C for 16 h light period and 22 ◦C for 8 h dark
period) separately or in combination. Regenerated plants (treated with chemo- and thermotherapy)
were indexed for PNRSV by DAS-ELISA and RT-PCR. An amplified product of 785 bp was obtained
by RT-PCR in PNRSV-infected plants. Virazole at a concentration of 20 mg/l was found to be more
effective (30 and 20% of PNRSV-free plants as indexed by ELISA and RT-PCR, respectively) in
comparison to the other chemicals. Thermotherapy for 25 days gave 35 and 25% PNRSV-free plants
as indexed by DAS-ELISA and RT-PCR, respectively. A combination of both treatments gave a good
number of PNRSV-free plants (67.5 and 57.5% as indexed by DAS-ELISA and RT-PCR, respectively).
At higher concentrations all three chemicals were found to be toxic. Thermotherapy for more
than 25 days caused browning of leaves and shoots died.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/905/1/science.pdf
Verma, N and Raja, Ram and Zaidi , A A (2005) In vitro production of Prunus necrotic ringspot virus-free begonias through chemo- and thermotherapy. Science Horticulture, 103. pp. 239-247.
 
Relation http://ihbt.csircentral.net/905/