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FIRST REPORT OF APPLE STEM GROOVING VIRUS FROM POME AND STONE FRUITS IN INDIA

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Title FIRST REPORT OF APPLE STEM
GROOVING VIRUS FROM POME AND
STONE FRUITS IN INDIA
 
Creator Negi, A
Rana, T
Kumar, Y
Hallan, Vipin
Zaidi , A A
 
Subject Plant sciences
Plant viruses
 
Description Apple stem grooving virus (ASGV), the type member of
the genus Capillovirus, induces severe pitting and grooving
of the xylem, brown line and graft union abnormalities, reduced
vigour of the canopy and an overall decline in susceptible
Malus species (Yoshikawa, 2000). It also infects
apricot, cherry, and pear. Infection rate in apple can be as
high as 80-100% and yield losses my reach 40%. During a
survey in the temperate fruit-growing Indian districts of
Himachal Pradesh (Shimla, Solan and Palampur) and Jammu
& Kashmir (Srinagar), preliminary evidence for the
presence of ASGV was obtained by DAS-ELISA, using a
commercial kit (Adgen Phytodiagnostics, UK). To confirm
the record, total RNA was extracted using RNeasy Plant
Mini kit (Qiagen, Germany) from one of the positive apple
samples (cv. Starkrimson) which had reacted strongly in
ELISA. PCR primer pair ASGV6396 (reverse), 5’-CTG
CAA GAC CGC GAC CAA GTT T-3’ and ASGV5641
(forward), 5’-ATG AGT TTG GAA GAC GTG CTT C-
3’, yielding an amplicon of 755 base pairs (bp) was used to
amplify the complete ASGV coat protein (CP) gene, as described
by Nickel et al. (2001). ASGV was detected in
eleven different cultivars of apple, nectarine, plum, cherry,
quince and apricots by DAS-ELISA and dot blot hybridization
using the cloned CP of the present isolate as a
probe. Multiple alignment of CP sequences of the Indian
ASGV isolate showed 100% identity at the amino acid level
with a Brazilian isolate from apple (accession No.
AF438409) whereas identities of 95-98% and 95-100%
were found with a citrus strain (Citrus tatter leaf virus) and
other isolates, respectively. To our knowledge, this is the
first report of ASGV infection to pome and stone fruit
trees in India.
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://ihbt.csircentral.net/924/1/NEGI501.pdf
Negi, A and Rana, T and Kumar, Y and Hallan, Vipin and Zaidi , A A (2009) FIRST REPORT OF APPLE STEM GROOVING VIRUS FROM POME AND STONE FRUITS IN INDIA. Journal of Plant Pathology, 91 (2). p. 501.
 
Relation http://ihbt.csircentral.net/924/